Everything About Human IL-4 ELISA Kit
The Human Interleukin 4 ELISA Kit (IL-4) is used to measure and quantify human IL-4 levels in biological fluids, such as plasma and cell culture supernatants. Th2 cells and NK cells produce IL-4, as well as g/d T cell, eosinophils. Mast cells and activated basophils also make it.
IL-4 causes Ig isotype switching to B cells, regulates Th2 cell differentiation into Th2 cells, and, in combination with TNF recruits T cells as well as eosinophils at sites of inflammation. In human IL-4 ELISA Equipment, sandwich ELISA takes place, where samples are added to ELISA strips plates coated with specific capture antibodies for the cytokine.
The cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. The color is created by adding the chromogenic enzyme substrate tetramethylbenzidine to the sample.
It has an intensity that is directly proportional with the amount of cytokine present in it. Comparing the serial dilution of a cytokine standard to determine the concentration of the cytokine, parallel analysis is used. This assay can only recognize natural and recombinant Hu IL-4. The microplate has wells for the target-specific antibody.
These wells can then be filled with samples, standards, and controls that bind to the captured (immobilized) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal.